To introduce students to the rapidly growing in recent years "Pharmaceutical Biotechnology" to gain knowledge on the basic concepts, technologies and applications of molecular biotechnology in the production of experimental approaches using drugs, enzymes, genes, GM microorganisms and plants.
Course Content (Syllabus)
Introduction to molecular biotechnology; biodiagnostic technologies; applications of molecular biology and biotechnology techniques in plant physiology and genetic (molecular) improvement; bioinformatics. Genomic and metagenomic sciences. Plant tissue culture (totipotency, clonal particle variability, regeneration).
Basic principles of genetic engineering of plants: applications and necessity; methods and carriers of genetic transformation of plants and transfer DNA: genetic vectors (Agrobacterium tumefaciens, Agrobacterium rhizogenes, biolistic method, immersion in a vacuum, etc).
Genetically modified plants: selection of transgenic plants; production of homozygous lines and evaluation.
Genetic modification of pharmaceutical plants and production of secondary metabolites and biopharmaceuticals.
Production of hairy roots: applications for the production of biopharmaceuticals.
Applications of functional genomics to enhance the production of known and novel secondary metabolites and biopharmaceuticals in plant cells.
Molecular farming, medical molecular agriculture (vaccines and edible vaccines, antibodies, proteins, etc.).
Bioethics and genetically modified plants and foods.