Separation Methods in Chemical Analysis

Course Information
TitleΜΕΘΟΔΟΙ ΔΙΑΧΩΡΙΣΜΟΥ ΣΤΗ ΧΗΜΙΚΗ ΑΝΑΛΥΣΗ / Separation Methods in Chemical Analysis
Cycle / Level1st / Undergraduate
Teaching PeriodWinter
Course ID40001097

Class Information
Academic Year2016 – 2017
Class PeriodWinter
Faculty Instructors
Class ID
Mode of Delivery
  • Face to face
Digital Course Content
Learning Outcomes
The students will get familiar with separation analytical techniques and they will be informed on the use of the instruments, the applications, the advantages and the disadvantags of each technique. They will be qualified on the sample preparation as well as on the method development.
General Competences
  • Apply knowledge in practice
  • Retrieve, analyse and synthesise data and information, with the use of necessary technologies
  • Work in teams
  • Respect natural environment
  • Advance free, creative and causative thinking
Course Content (Syllabus)
Theoretical aspects of separation techniques. Sample preparation techniques. Preconcentration. Solid phase extraction. Supercritical fluid extraction. High Pressure Liquid Chromatography. Capillary electrophoresis. Ion chromatography. Gas chromatography. Hyphenated techniques GC-MS, LC-MS, preparative chromatography, chiral chromatography. Column switching techniques. Applications of separation techniques. Troubleshooting in HPLC. Principles, errors and interferences. Analytical method validation. Real sample analysis. Determination of caffeine, theobromine, theophylline, mefenamic acid, vitamins, paracetamol, in pharmaceuticals and biological samples, after solid phase extraction. Determination of inorganic ions by ion chromatography. Calculation of chromatographic parameters.
Educational Material Types
  • Slide presentations
  • Multimedia
  • Book
Course Organization
Laboratory Work
Student Assessment
Student Assessment methods
  • Written Exam with Multiple Choice Questions (Summative)
  • Written Exam with Short Answer Questions (Summative)
  • Written Exam with Extended Answer Questions (Summative)
  • Written Assignment (Summative)
  • Written Exam with Problem Solving (Summative)
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