Nucleic acids structure and function

Course Information
TitleΔομή και Λειτουργία Νουκλεϊνικών οξέων / Nucleic acids structure and function
Cycle / Level2nd / Postgraduate
Teaching PeriodWinter
Course ID40003159

Programme of Study: PPS of School of Chemistry (2014-2015)

Registered students: 4
OrientationAttendance TypeSemesterYearECTS
BiochemistryCompulsory Course1110

Class Information
Academic Year2016 – 2017
Class PeriodWinter
Faculty Instructors
Weekly Hours3
Class ID
Course Type 2016-2020
  • Scientific Area
Course Type 2011-2015
Specific Foundation / Core
Mode of Delivery
  • Face to face
Digital Course Content
Language of Instruction
  • Greek (Instruction, Examination)
Required Courses
  • ΜΧ031 Nucleic acids structure and function
  • ΜΧ032 Structure and function of proteins
Learning Outcomes
Upon successful completion of this course, students will have acquired research thinking and the necessary they need the understanding and importance of nucleic acids and the functions they perform
General Competences
  • Retrieve, analyse and synthesise data and information, with the use of necessary technologies
  • Work in teams
  • Work in an interdisciplinary team
  • Advance free, creative and causative thinking
Course Content (Syllabus)
Chemistry and metabolism of nucleic acids. Principles of recombinant DNA. Structure of nucleic acids and nucleotides (ribonucleotides, deoxyribonucleotides). DNA as a storage molecule of genetic information. The primary role of RNA. β-glucositic bond of nucleotides. Biosynthesis and degradation of nucleic acids. Stability of nucleic acids against hydrolysis. Catalyzed-mechanism of basic hydrolysis. Secondary structures of DNA and RNA.– Forms of DNA helix. Double helix, major and minor groove, Α-DNA, Β- DNA, Ζ-DNA, triple helix and its biological role. DNA topoisomers, topoisomerases type I and II, gyrases and mechanisms of action. Helicases and mechanisms of action. Interaction of nucleic acids with other molecules. Intercalators of double helix and their effects. Properties of nucleic acids. Chromatographic and electrophoretic behavior, and separation of nucleic acids. Supercoiled-relaxed DNA. Denaturation-renaturation of DNA. Affinity chromatography of nucleic acids and their applications. Control of Gene experession An overview of gene control, Gene regulatory proteins, Regulation of transcription in procaryotic cells-operons, Regulation of transcription in eukaryotic cells, Posttranscriptional control, Alternative RNA splicing, RNA transport and stability in eukaryotic cells, miRNAs – siRNAs Cell communication Cell response to extracellular signals, Signaling through G-protein-linked cell surface receptors, Signaling through enzyme-linked cell surface receptors, Ion channels DNA damage, repair and recombination DNA damage, DNA damage and cancer, DNA repair, DNA recombination
Nucleic acids, Metabolism, Fuctions
Educational Material Types
  • Slide presentations
Course Organization
Reading Assigment301.2
Written assigments301.2
Student Assessment
Student Assessment methods
  • Written Exam with Extended Answer Questions (Formative, Summative)
  • Written Assignment (Formative)
  • Performance / Staging (Formative)
Last Update