Course Content (Syllabus)
Historical overview. Cell: Procaryotic and eukaryotic cells. Morphology. Chemical components of cells. Microorganisms. Important cell types. Cell cultures (animal, plant and microbial). Kinetics of cell growth-Equations. Methods for determination of cell growth. Enzymes. Kinetics of enzyme-catalyzed reactions. Michaelis-Menten equation. Briggs-Haldone kinetics. Evaluations of parameters in the Michaelis-Menten equation. Effect of pH and tempwrature on Enzyme kinetics. Order of enzyme-catalyzed reaction. Inhibition. Reversible inhibition. Competitive and non competitive inhibition. Immobilized enzymes and applications: Methods for immobilization. Examples. Applications of immobilized enzymes in industry. Production of L-amino acids with immobilized. a-amino acylase, β-galactosidase for hydrolysis of lactose, semi synthetic penicillins. Commercial immobilized enzymes. Sterilization: Methods of sterilization. Heat, chemical reagents, mechanical methods, ultrasound. Sterilization by heating. Continuous and non continuous sterilization. Bioreactors: Types of bioreactors. Productivity and designing of bioreactors. Factors influencing the operation of a bioreactor. Bioseparations-Dowbstream processing: General. Broth handling Seperation of liquid-solid. Filtration, Centrifugation. Cell lysis (Chemical, Enzymic and Physical methods). Product recaovery: Extraction. Adsorption. Product purification: Precipitation, Electrophoresis, Chromatography ion exchange, affinity and gell permeation chromatography). Ultrafiltration. Biotechnological applications. Synthesis and modification of polymers. Proteins and polypeptides Oligo- and polysaccharides. Degradation of starch by enzymes. Single cell proteins. Biosensors of proteins.
Keywords
Microorganisms, Immobilized enzymes, Bioseparations-Dowbstream processing, Biotechnological applications