After completing the course, the student should be able to:
- Identify and describe the national and European legislation which regulate the scientific use of animals
- Demonstrate a comprehensive understanding of the principle of the 3Rs, list examples of how the 3Rs can be implemented in research projects
- Describe the severity classification system
- Describe the principle of implementing early humane endpoints and how this implementation can influence animal health and welfare during the course of an experiment
- Describe the basic biology of the relevant animal species, including physiology, reproduction and behavior
- Describe the environmental factors of importance for maintaining an appropriate health status for the animals
- Describe the principles of humane killing and list appropriate euthanasia methods for the relevant animal species
- Recognize that the choice of a euthanasia method may influence the scientific outcome
- Describe appropriate methods and principles for handling animals, and describe common techniques / procedures, including administration and sampling techniques
- Describe the variables affecting significance, including the meaning of statistical power and the "p-value"
- Indicate some of the problems associated with pain recognition and pain management in animals
Course Content (Syllabus)
1st-2nd hour Introduction. Laboratory animal species. The use of laboratory animals in biomedical research. Legislation for the well-being of animals during experimental protocols. Historical background. Future perspectives.
3rd-4th hour Physiology of the reproductive system. Management of animal reproduction in the facility.
5th-6th hour Physiology of the digestive system. Nutritional requirements. Physiopathology of digestive disorders.
7th-8th hour Physiology of the endocrine system. Physiology of the circulatory system, the blood and haemopoietic tissues. Physiology of the immune system.
9th-10thhour Laboratory animal facilities. Cages and Housing. Physiopathology of management and housing-related diseases. Stress, pain, behavior. Memory and learning. Animal welfare.
11th-12th hour Experimental protocol design, applications of experimental protocols in accordance with species characteristics and the experimentation prerequisites. Animal handling and biological material sampling. Safety.
4 hours Laboratory animal handling
Additional bibliography for study
-The handbook of experimental animals: The Laboratory Rat, Academic Press, 2000, editor Georg J Krinke
-The Experimetal Animal in Bimedical Research (Volumes I, II) CRC Press, 1995, editors Rollin E Bernard, Kesel Lynne M
-Fundamentals of Laboratory Animal Science: CRC press 2017, Enqui Liu and Jianglin Fan (eds).