Tissue Culture

Course Information
TitleΙΣΤΟΚΑΛΛΙΕΡΓΕΙΑ 1 / Tissue Culture
CodeΕΟΝ701
FacultyAgriculture, Forestry and Natural Environment
SchoolAgriculture
Cycle / Level2nd / Postgraduate
Teaching PeriodSpring
CommonYes
StatusActive
Course ID420000743
Class Information
Academic Year2018 – 2019
Class PeriodSpring
Faculty Instructors
Weekly Hours3
Class ID
600121048
Course Type 2016-2020
  • Scientific Area
Course Type 2011-2015
Specific Foundation / Core
Mode of Delivery
  • Face to face
Digital Course Content
Erasmus
The course is also offered to exchange programme students.
Language of Instruction
  • Greek (Instruction, Examination)
  • English (Examination)
Prerequisites
Required Courses
  • ΒΑΖΝ702 Cytogenetics and Plant Evolution
  • 011Υ PLANT PHYSIOLOGY
  • 024Ε GENETICS
  • 116Υ PLANT BREEDING
  • 610Υ PLANT BREEDING
  • 613Υ AGRICULTURAL GENETICS
  • 658Ε GENETICS AND PLANT BREEDING (PRACTICALS)
Learning Outcomes
1. will understand the clonal propagation systems and mechanisms of plant (asexual propagation) 2. be familiar with the tissue culture techniques and methods 3. will learn about the practical aplication of tissue culture in plant breeding (in vitro production of hybrids, haploids) 4. they will learn the method of in vitro selection for generating variability, micropropagation and conservation of genetic material 6. will be informed about the management of a tissue culture laboratory and the production of mas propagation material
General Competences
  • Apply knowledge in practice
  • Retrieve, analyse and synthesise data and information, with the use of necessary technologies
  • Adapt to new situations
  • Work in teams
  • Work in an international context
  • Work in an interdisciplinary team
  • Generate new research ideas
  • Respect natural environment
  • Advance free, creative and causative thinking
Course Content (Syllabus)
Introduction history • Laboratory requirements • Nutrient media • Nutrient medium sterilization, Plant material disinfestation (explants) • Organogenesis • Somatic embryogenesis and zygotic embryo rescue • Virus-free plants(meristem culture)  Haploid production (anther and pollen culture)  Protoplast isolation and culture  Protoplast fusion (somatic hybridisation)  Somaclonal variation  Germplasm storage (cryopreservation)  Micropropagation of horticultural and agronomic crops and forestry species
Keywords
Tissue culture, clonary propagated plants, asexual breeding, in vitro selection, germplasm preservation
Educational Material Types
  • Notes
  • Slide presentations
Use of Information and Communication Technologies
Use of ICT
  • Use of ICT in Course Teaching
  • Use of ICT in Communication with Students
Course Organization
ActivitiesWorkloadECTSIndividualTeamworkErasmus
Lectures190✓✓
Laboratory Work12✓✓✓
Reading Assigment20✓✓
Field trips and participation in conferences / seminars / activities7✓✓
Written assigments20✓✓
Exams20✓✓
Total269
Student Assessment
Description
Presence and Participation in the class Written exam (7th or 8th weeks of lecture)20% Term paper and Presentation of assignment 20% Final written exam 60%
Student Assessment methods
  • Written Exam with Multiple Choice Questions (Summative)
  • Written Exam with Short Answer Questions (Formative, Summative)
  • Performance / Staging (Formative, Summative)
Bibliography
Additional bibliography for study
Α. Οικονόμου: Σημειώσεις Παραδόσεων Karl-Hermann Neumann • Ashwani Kumar Jafargholi Imani (2009). Plant Cell and Tissue Culture - A Tool in Biotechnology Basics and Application, Springer Victor M. Loyola-Vargas, Neftali Ochoa-Alejo (2012) Plant Cell Culture Protocols. Humana Press OLUF L. GAMBORG (2002).PLANT TISSUE CULTURE. BIOTECHNOLOGY. MILESTONES. In Vitro Cell. Dev. Biol.—Plant 38:84–92 Maria Antonietta Germana (2011). Anther culture for haploid and doubled haploid production Plant Cell Tiss Organ Cult 104:283–300 Pablo Salas ,Alba Rivas-Sendra, Jaime Prohens, Jose´ M. Seguı´-Simarro (2012). Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica 184:235–250 Adam Matkowski Biotechnology Advances 26 (2008) Plant in vitro culture for the production of antioxidants — A review. 548–560 M. J. M. Smulders ,G. J. de Klerk (2011) Epigenetics in plant tissue culture Plant Growth Regul 63:137–146 Ammara Maryam, Raima Tariq, Samra chuadhary, Rabia Azmat, Sonia Javed and Sadia Khanam. ( 2014)A Review: Role of Tissue Culture (in-vitro) techniques in The conservation of rare and Endangered Species. Pacific Journal of Life Sciences Vol. 2(2): 93-103 άρθρα(papers)
Last Update
13-11-2015