Biotechnology

Course Information
TitleΒιοτεχνολογία / Biotechnology
CodeBT5
FacultyEngineering
SchoolChemical Engineering
Cycle / Level1st / Undergraduate
Teaching PeriodWinter
CommonNo
StatusActive
Course ID20002619

Programme of Study: PPS Tmīmatos CΗīmikṓn Mīchanikṓn (2019-sīmera)

Registered students: 0
OrientationAttendance TypeSemesterYearECTS
KORMOSCompulsory Course535

Class Information
Academic Year2018 – 2019
Class PeriodWinter
Faculty Instructors
Weekly Hours4
Class ID
600125371
Type of the Course
  • Background
Course Category
General Foundation
Mode of Delivery
  • Face to face
Digital Course Content
Erasmus
The course is also offered to exchange programme students.
Language of Instruction
  • Greek (Instruction, Examination)
  • English (Instruction, Examination)
Learning Outcomes
The students will widen further their knowledge of Biotechnology, in particular the role of microorganisms, genetic engineering, enzymes and bioseparations. This knowledge is considered necessary for a Chemical Engineer, since the application of biotechnology is often required both in traditional chemical engineering and in modern biochemical engineering and biomedical applications.
General Competences
  • Apply knowledge in practice
  • Retrieve, analyse and synthesise data and information, with the use of necessary technologies
  • Adapt to new situations
  • Make decisions
  • Work autonomously
  • Work in an interdisciplinary team
  • Generate new research ideas
  • Design and manage projects
  • Respect natural environment
  • Advance free, creative and causative thinking
Course Content (Syllabus)
Historical overview. Cell: Procaryotic and eukaryotic cells. Morphology. Chemical components of cells. Microorganisms. Important cell types. Cell cultures (animal, plant and microbial). Kinetics of cell growth-Equations. Methods for determination of cell growth. Enzymes. Kinetics of enzyme-catalyzed reactions. Michaelis-Menten equation. Briggs-Haldone kinetics. Evaluations of parameters in the Michaelis-Menten equation. Effect of pH and tempwrature on Enzyme kinetics. Order of enzyme-catalyzed reaction. Inhibition. Reversible inhibition. Competitive and non competitive inhibition. Immobilized enzymes and applications: Methods for immobilization. Examples. Applications of immobilized enzymes in industry. Production of L-amino acids with immobilized. a-amino acylase, β-galactosidase for hydrolysis of lactose, semi synthetic penicillins. Commercial immobilized enzymes. Sterilization: Methods of sterilization. Heat, chemical reagents, mechanical methods, ultrasound. Sterilization by heating. Continuous and non continuous sterilization. Bioreactors: Types of bioreactors. Productivity and designing of bioreactors. Factors influencing the operation of a bioreactor. Bioseparations-Dowbstream processing: General. Broth handling Seperation of liquid-solid. Filtration, Centrifugation. Cell lysis (Chemical, Enzymic and Physical methods). Product recaovery: Extraction. Adsorption. Product purification: Precipitation, Electrophoresis, Chromatography ion exchange, affinity and gell permeation chromatography). Ultrafiltration. Biotechnological applications. Synthesis and modification of polymers. Proteins and polypeptides Oligo- and polysaccharides. Degradation of starch by enzymes. Single cell proteins. Biosensors of proteins.
Keywords
Microorganisms, Immobilized enzymes, Bioseparations-Dowbstream processing, Biotechnological applications
Educational Material Types
  • Notes
  • Book
Use of Information and Communication Technologies
Use of ICT
  • Use of ICT in Communication with Students
Course Organization
ActivitiesWorkloadECTSIndividualTeamworkErasmus
Lectures50
Reading Assigment90
Tutorial7
Exams3
Total150
Student Assessment
Student Assessment methods
  • Written Assignment (Formative, Summative)
  • Oral Exams (Formative, Summative)
  • Written Exam with Problem Solving (Formative, Summative)
Bibliography
Course Bibliography (Eudoxus)
1. Μ. Λιακοπούλου-Κυριακίδου, 'Βιοτεχνολογία με στοιχεία Βιοχημικής Μηχανικής', Εκδόσεις Ζήτη
Last Update
20-06-2018