Course Information
FacultyHealth Sciences
Cycle / Level1st / Undergraduate
Teaching PeriodWinter
Course ID180000083

Class Information
Academic Year2018 – 2019
Class PeriodWinter
Faculty Instructors
Weekly Hours5
Class ID
Mode of Delivery
  • Face to face
Digital Course Content
Language of Instruction
  • Greek (Instruction, Examination)
Learning Outcomes
The course enables students not only to identify medicines(active ingredients) but also to quantify them in their pure form, in pharmaceutical formulations and in biological liquids. Ultraviolet spectrophotometry is considered a very useful technique in Pharmaceutical Analysis because it is highly sensitive (due to electronic excitations).Theory of electronic spectroscopy- Orbitals involved in electronic transitions. Furthermore, identification of medicines is carried out on the basis of their various chromophore groups(the chromophore concept) because they provide (give) specific spectrum ( which is characterized from its fine structure: maxima, minima, diversions, shoulders, width of electronic excitations,that is,width in the spectrum where the electronic promotions occur, values of specific absorption coefficient, A 1%, 1cm, ratios of absorption intensities in specific maxima, minima or combination of the them, pH solution etc). This method, because of the above mentioned advantages, can be combined with High-Performance Liquid Chromatography in order to detect, identify and quantify the medicines in the presence of the various impurities and metabolites. Ultraviolet spectrophotometry is described extensively in various Pharmacopoeias (Greek, European etc) as a useful means for the identification, the purity control and the quantitative determinations of various medicines in pharmaceutical formulations. This kind of knowledge is considered fundamental and essential for a possible future professional employment of a Pharmacy graduate in a Pharmaceutical Analysis Laboratory (in addition it includes instrumentation and sampling: dispersive instruments-photomultiplier detectors-rapid scan ultraviolet/visible spectrometers-linear photodiode arrays –Fourier Transform ultraviolet/visible spectrophotometers etc.)
General Competences
  • Apply knowledge in practice
  • Retrieve, analyse and synthesise data and information, with the use of necessary technologies
  • Work autonomously
  • Work in teams
Course Content (Syllabus)
Principles of instrumental methods. Classification of instrumental analytical methods. General characteristics of instrumental methods that are used in Pharmaceutical Analysis. Energy and the electromagnetic spectrum. The nature of the radiated energy(ultraviolet/visible is a form of energy ,which can be described by two complementary theories: the wave theory and the corpuscular theory. Neither of these theories alone can completely account for all the properties of light : some properties are best explained by the wave theory ,and others by the corpuscular theory.The wave theory supports the propagation of light by light waves involves both electric and magnetic forces). Interactions of electromagnetic radiation with matter. Classification of spectroscopic analytical techniques which can be applied in Pharmaceutical Analysis. Generally about spectroscopic methods. Ultraviolet spectrophotometry: Absorption and emission spectra. Principles methods. Study of ultraviolet spectrum of various representative organic molecules(called chromophores) which are of particular interest in Pharmaceutical Analysis(spectra of benzene ,aniline, pyridine ,phenol and diphenols ,barbituric acid and its derivatives ,xanthines,hormones ,corticosteroids,anti-inflammatory ,anti-histamine). Quantifications through ultraviolet spectrophotometry: The importance of specific absorption coefficient in quantitative analysis. Spectophotometric quantifications of active ingredients in different formulations. Polar and non- polar solvents. Stock solutions. Quantifications through derivatisation. Flamespectrophotometry and spectrophotometry of individual absorption: flames, burner nebulisers. Transmission Flamespectrophotometry. Individual absorption spectrophotometry. Instrumentation, sensitivity and detection limit. Applications
Educational Material Types
  • Notes
  • Slide presentations
  • Interactive excersises
Use of Information and Communication Technologies
The laboratory content of the module has been enriched with electronic educational material that is special software in CD-ROM format which contains the spectra of some representative medicines as well as their spatial configurations, that is, the chemical structure in space (bonds length, ring arrangement, various bonds angles) so that the relation of the structure of the medicine with the relevant spectrum to be obvious. Moreover, a large part of the laboratory practice is done by the students with the application of a special UVPC programme. The lectures of the modules are enriched by multimedia use (power point presentations, videos etc).
Course Organization
Laboratory Work391.3
Written assigments280.9
Student Assessment
Once the laboratory course is successfully completed with a pass mark and the laboratory notebook is handed in corrected, each student with his/her team should hand in a paper on an earlier assigned topic (the paper should be corrected). At the end of the academic year, students have to undergo oral exams. After having completed the above mentioned obligations, students can participate in the written exams of the pharmaceutical analysis modules. Cooperativeness and interpersonal relationship with the instructor plays an important role in the student assessment.
Student Assessment methods
  • Written Exam with Short Answer Questions (Formative)
  • Written Exam with Extended Answer Questions (Formative)
  • Written Assignment (Formative)
  • Oral Exams (Formative)
  • Written Exam with Problem Solving (Formative)
  • Labortatory Assignment (Formative)
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