Learning Outcomes
To introduce students to the rapidly developing, in recent years, area of «Pharmaceutical Biotechnology» and familiarize them with the basic concepts, technologies and applications of molecular biotechnology in the production of pharmaceuticals, using enzymes, genes, genetically modified organisms and plants.
Course Content (Syllabus)
- Molecular Biotechnology (Principles and applications)
- Gene identification, cloning and expression/protein production
- Recombinant protein expression systems (bacteria, yeasts, plant cells/plants, cells from multicellular eukaryotes, transgenic animals)
- Issues related to quantity, quality (protein folding, and activity) and purification of recombinant proteins.
- Protein transduction technologies
- Formulation of therapeutic proteins
- Pharmacokinetics - Pharmacodynamics and immunogenicity of therapeutic proteins
- Hematopoetic and growth factors, interferons, interleukins, insulins, growth hormone, and other peptide hormones, recombinant blood coaggulation proteins and thrombolytics
- Therapeutic and diagnostic antibodies (structure, development, modifications)
- Vaccines
- Genomics and other -omics technologies
- Gene therapy
- Cell therapies and regenerative medicine
- Biotherapeutics and biosimilars
- Regulatory affairs on the production, quality, and licensing of biotherapeutics
- Bioethics and issues related to the use of genetically modified organisms used in biotherapeutics production
Course Bibliography (Eudoxus)
1. D.J.A. Crommelin, R.D. Sindelar, B. Meibohn, «Φαρμακευτική Βιοτεχνολογία» (3η έκδοση, 2011) Επιμέλεια Ελληνικής Έκδοσης: Α.Σ. Τσιφτσόγλου, Γ. Σωτηροπούλου
2. Watson, J. D., Myers, R.M., Caudy, A.A., Witkowski, J.A. ΑΝΑΣΥΝΔΥΑΣΜΕΝΟ DNA Συγγραφείς: James D. κ.α. Έκδοση: 3/2007, Ακαδημαϊκές Εκδόσεις Ι. Μπάσδρα & Σια.