Course Content (Syllabus)
Theoretical aspects of separation techniques. Sample preparation techniques. Preconcentration. Solid phase extraction. Supercritical fluid extraction. High Pressure Liquid Chromatography. Capillary electrophoresis. Ion chromatography. Gas chromatography. Hyphenated techniques GC-MS, LC-MS, preparative chromatography, chiral chromatography. Column switching techniques. Applications of separation techniques. Troubleshooting in HPLC. Principles, errors and interferences. Analytical method validation. Real sample analysis. Determination of caffeine, theobromine, theophylline, mefenamic acid, vitamins, paracetamol, in pharmaceuticals and biological samples, after solid phase extraction. Determination of inorganic ions by ion chromatography. Calculation of chromatographic parameters.
Separation techniques, HPLC, GC, CE, Ion chromatography, Troubleshooting, Method validation