Upon successful completion of the course, students will be expected to understand the basic principles of modern Molecular Biology techniques, as well as to get in touch with biotechnological applications of enzymes.
Course Content (Syllabus)
Growth of microorganisms. Improving strains with commercial value. Selection and isolation of strains. Site directed mutagenesis. Principles of recombinant DNA, protein engineering. Restriction nucleases, DNA sequence analysis, Nucleic acid hybridization, DNA cloning, DNA engineering, Genetic engineering, Applications of protein engineering, Fusion proteins.
Enzyme structural modification by chemical methodologies. Methods for enzyme immobilization. Enzyme binding to insoluble vectors. Intermolecular enzyme coupling. Enzyme encapsulation in liposomes or hydrophobic polymeric matrices. Properties of immobilized enzymes. Immobilization of coenzymes, polyenzymic systems and cell immobilization. Downstream processing for enzymes of industrial value.Bioreactors.Characterization of biotechnological processes. Commercial production of primary and secondary metabolites. Industrial enzymes. Utilization of cellulose-lignocellulose. Microbial recovery of metals. Biochemical electrodes.
Molecularbiology, enzymes, immobilization, biotechnological processes
Course Bibliography (Eudoxus)
1) Δ.Α. Κυριακίδη, Βιοτεχνολογία, Εκδόσεις ΖΗΤΗ 2000
2) Ι. Κλώνης, Ενζυμική Βιοτεχνολογία, Πανεπιστημιακές Εκδόσεις Κρήτης, Έτος Έκδοσης: 2010, ISBN: 9789605243043