Biochemistry I

General Foundation

The course is also offered to exchange programme students.

Upon successful completion of the course students will have acquired basic knowledge about the structure and function of proteins and enzymes, hence the functioning of living organisms at the molecular level. They will gain basic knowledge of the nucleic acids (DNA, RNA), the biosynthesis of nucleic acids and their catabolism. Upon completion of this course they will have learned the genetic code that governs all living organisms, from the simplest to the most complex that is the man. They'll also understand the mechanism of protein synthesis and how it is regulated, covalent modifications of proteins and its formation in higher configurations. Finally they will know the technology of recombinant DNA, which will be taught in more detail in other lessons.

Introduction Water (physical properties and hydrogen bonding of water, structure of liquid water, The ion product of water: The pH scale, acids and bases, The Fitness of the aqueous environment for living organisms). Amino acids-Proteins Acid- base properties of amino acids, the amino acids as building blocks of proteins, common amino acids, rare amino acids, non protein amino acids. The stereochemistry of amino acids and the absorption spectra. Amino acid reactions of amino groups, carboxyl groups and residue groups. Classification of proteins, Protein structure (covalent backbone and amino acid sequence, peptides, primary, secondary, tertiary, quaternary structure, higher conformation of proteins). Biological role of proteins, structural and functional proteins, protein denaturation, structure function relationship of proteins. Enzymes Naming and classification of enzymes , Kinetics , enzyme cofactors, coenzymes, cytochromes, inhibition of enzymic reactions, enzymic specificity, enzyme substrate complexes and covalent enzyme-substrate compounds , enzyme function regulation, isoenzymes Nucleic acids Nucleic acids and primary structure. Secondary structure of nucleic acids, DNA, RNA properties in solution, the biosynthesis of nucleotides, nucleic acid catabolism. Recombinant DNA ,DNA replication , transcription and repair. Protein synthesis The genetic code, the mechanism of protein synthesis, regulation of protein synthesis, covalent modifications ,higher conformational structures of proteins and subcellular localization. Cellular defense against viral components.Recombinant DNA.

biochemistry, amino acids, enzymes, nucleic acids, genetic code, mechanism of protein synthesis, regulation of protein synthesis

17-03-2021