Most of the reaction in the living systems (see Biochemistry) can not function without the interference of enzymes. By the end of this course the students will know: a) the nature, quantitative determination, purification, isolation and specificity of the enzymes b) the mechanisms and kinetic of enzyme reactions as well as the factors that affect them, and c) the regulation of biosynthesis and activity.
Course Content (Syllabus)
Νomenclature and Classification of Enzymes: Structure and role of enzymes-Criteria of enzymatic reactions – Quantitative determination of enzymes-Selection of method for detection of enzymatic activity-Sources of enzymes-Methods of extraction and purification of enzymes-Mechanisms of enzymatic reactions-Reactions of oxidoreduction-
Reactions of groups transfer-Hydrolytic and non hydrolytic reactions-Synthetic reactions-Kinetics of enzymatic reactions-Constants of Michaelis Menten rections-Factors affecting enzymatic reactions-Enzymatic reactions with many substrates-Active site of enzymes-Enzymes Inhibitors-Activators-Allosteric phenomenon and biological significance-Stereoselectivity of enzymes-Enzymes activity and post-translational modification of enzymes- Regulation of enzymes biosynthesis in bacteria and animal cells. Enzymes activity in non-conventional media
enzymes, enzymatic reactions, kinetics, allosteric phenomena
Course Bibliography (Eudoxus)
Γεωργάτσος Ι., Γιουψάνης, Κυριακίδης Δ. (2001). Ενζυμολογία. Εκδόσεις Ζήτη. Θεσσαλονίκη
1α. Ενζυμολογία: Ι.Γ. Γεωργάτσος- Τ.Α. Γιουψάνης- Δ.Α. Κυριακίδης
(Εκδόσεις: Ζήτη), Θεσσαλονίκη, 2001.
2. Ενζυμολογία: Ι. Κλώνης (Γεωπονικό Παν/μιο Αθηνών-Εκδόσεις: Κρήτης, Αθήνα: ΄Εμβρυο, 2007.