GENETICS AND PLANT BREEDING (PRACTICALS)
| Title | ΓΕΝΕΤΙΚΗ ΚΑΙ ΒΕΛΤΙΩΣΗ ΦΥΤΩΝ (ΕΡΓΑΣΤΗΡΙΟ) / GENETICS AND PLANT BREEDING (PRACTICALS) |
| Code | 658Ε |
| Faculty | Agriculture, Forestry and Natural Environment |
| School | Agriculture |
| Cycle / Level | 1st / Undergraduate, 2nd / Postgraduate |
| Teaching Period | Winter/Spring |
| Common | No |
| Status | Active |
| Course ID | 420001548 |
| Academic Year | 2021 – 2022 |
| Class Period | Winter |
| Faculty Instructors | |
| Class ID | 600195170
|
Course Type 2016-2020
- Scientific Area
- Skills Development
Course Type 2011-2015
Specific Foundation / Core
Mode of Delivery
- Face to face
Digital Course Content
- e-Study Guide https://qa.auth.gr/en/class/1/600195170
- At the Website of the School:
Language of Instruction
- Greek (Instruction, Examination)
- English (Examination)
Prerequisites
Required Courses
- ΒΑΖΝ722 Molecular Breeding
- 024Ε GENETICS
- 116Υ PLANT BREEDING
- 610Υ PLANT BREEDING
- 613Υ AGRICULTURAL GENETICS
- Ν539Ε MORIAKĪ GENETIKĪ
- Ν510Υ Plant Breeding
Learning Outcomes
At the end of the course, students will be able to:
1. Understand and discuss the importance and the application of the molecular and biotectechnological approaches in plant breeding.
2. Understand the importance of asexual micropropagation that have breeding aplications
3. The recognazed the differences between conventional plant breeding and aplication of genetic engineering
General Competences
- Apply knowledge in practice
- Retrieve, analyse and synthesise data and information, with the use of necessary technologies
- Adapt to new situations
- Work in teams
- Work in an interdisciplinary team
- Generate new research ideas
- Respect natural environment
- Advance free, creative and causative thinking
Course Content (Syllabus)
1st section
Laboratory Rules: (laboratory police, machine instructions / any mechanical or electrical apparatus/, preparation of solutions, protocols)
2nd section
Isolation of plant and plasmid DNA (material and methods)
3rd section
Measure Optical Density of DNA ,DNA cutting with restriction enzymes
4th section
DNA Gel electrophoresis: preparation of agarose gel, separation and analysis of macromolecules DNA
5th and 6th section
Polymerase Chain Reaction (PCR): Methods, DNA polymerase, oligonucleotide primers. Gel electrophoresis is performed for analytical purposes, often after amplification of DNA via PCR
7th section
In vitro culture and regeneration of explants: Methods and conditions, axilary shoot production, tissue culture applications in plant breeding
8th section
Genetic engineering in plants: genetic transformation via Α. tumefaciens and Gene gun technique and conditions,regeneration of transformants in selection media , confirmation of transformation
9th and 10th section
Chromosome (cytogenetic) analysis: the study of the structure and function of the cell, especially the chromosomes, analysis of G-banded
In Vivo Pollen Viability and Pollen Germination
11th section
Field trip: Application of Plant breeding methods in self and cross-fertilization plants. Genotype evaluation and selection of desirable traits
12th and 13th section
Crossing techniques
Keywords
Plant breeding, DNA technology, Polymerasy chain reaction, tissue culture, genetic transformation, transgene expression, Chromosome analysis, Pollen Viability and Germination, Crossing techniques
Educational Material Types
- Notes
- Slide presentations
- Book
Use of Information and Communication Technologies
Use of ICT
- Use of ICT in Laboratory Teaching
- Use of ICT in Communication with Students
Description
χρήση των Η/Υ, powerpoint
Course Organization
| Activities | Workload | ECTS | Individual | Teamwork | Erasmus |
|---|---|---|---|---|---|
| Laboratory Work | 30 | ✓ | ✓ | ✓ | |
| Fieldwork | 9 | ✓ | ✓ | ||
| Reading Assigment | 5 | ✓ | ✓ | ||
| Exams | 5 | ✓ | ✓ | ||
| Total | 49 |
Student Assessment
Description
100% written exams
Student Assessment methods
- Written Exam with Multiple Choice Questions (Formative)
- Written Exam with Short Answer Questions (Formative)
Bibliography
Course Bibliography (Eudoxus)
Ε. Γουλή-Βαβδινούδη και Μ. Κούτσικα-Σωτηρίου (2010) Εγχειρίδιο τεχνικής των διασταυρώσεων στα καλλιεργούμενα φυτά. Σύγχρονη Παιδεία, Θεσσαλονίκη
Τσαυτάρης Α., Ε. Νιάνιου-Ομπειντατ και Α. Πολύδωρος. Βελτίωση Φυτών. Θεσσαλονίκη: Σύγχρονη Παιδεία (Κωδικός Εύδοξος 148677)
Additional bibliography for study
Εργαστήρια Γενετικής και Βελτίωσης των Φυτών (Σημειώσεις),Τμήμα εκδόσεων, Πανεπιστημιακό Τυπογραφείο, ΑΠΘ
Last Update
13-11-2015
