Learning Outcomes
Upon completion of this course, students will be able to:
1. to have an in-depth knowledge of Molecular Biology principles
2. to understand the molecular functions of the cell and their control
3. to understand the techniques of Molecular Biology and their applications
4. to apply the techniques of Molecular Biology in foods (authenticity, detection of allergens, detection and identification of microorganisms etc)
Course Content (Syllabus)
1.Introduction in Molecular Biology. Key experiments. Nucleic acids. Structure and organization of genomes in cells.
2.DNA replication. DNA damage and repair.
3.The RNA structure, synthesis and processing. Protein synthesis.
4.Regulation of gene expression.
5.Methods in Molecular Biology: DNA isolation and properties. Gradient centrifugation. Electrophoresis of DNA.
6.Methods in Molecular Biology: Hybridization, DNA-RNA probes, Fluorescence, FISH, DΝΑ microarrays.
7.Methods in Molecular Biology: Polymerase Chain Reaction (PCR). Various types of PCR and applications in food science.
8.DNA sequencing, NGS.
9.Technology of recombinant DNA. Restriction enzymes. Vectors of clone and expression.
10.Gene transfer. Genetically modified organisms and foods. Safety, legislation, bioethics.
11.Study, detection and identification of food microorganisms with molecular methods.
12.Application of molecular methods in food fraud. Detection of food allergens
13.Nutrigenomics.
Keywords
Genomes of prokaryotic and eukaryotic cell, gene expression, genetically modified foods, molecular biology methods.
Additional bibliography for study
Tropp B.E (2015) Βασικές Αρχές Μοριακής Βιολογίας. Μετάφραση της 1ης Αγγλικής έκδοσης. Ακαδημαϊκές Εκδόσεις Ι. Μπάσδρα και ΣΙΑ ΟΕ, Αλεξανδρούπολη.
Watson, J.D., Witkowsky, J.A., Myers, R.M. and Caudy, A.A. (2007) Ανασυνδυασμένο DNA. Γονίδια και Γονιδιώματα - Μια Συνοπτική Παρουσίαση. Μετάφραση της 3ης Αμερικάνικης έκδοσης. Ακαδημαϊκές Εκδόσεις Ι. Μπάσδρα και ΣΙΑ ΟΕ, Αλεξανδρούπολη.