Learning Outcomes
Upon completion of the course, students will acquire basic knowledge about the holistic technologies of large-scale biological systems analysis (omics). They will become familiar with genome-metagenome, proteome and metabolome analysis methodologies and understand applications in plant breeding. They will understand the requirements and processes of applying holistic technologies to breeding programs and will be able to use these technologies in their research.
Course Content (Syllabus)
Genome-metagenome analysis and applications. Genomic manipulations, association characterization, genome-wide selection and refinement (GWAS), genetic characterization, microbial community analysis of environmental samples, microbe-plant interactions, microbial communities in plant pathology. Transcriptome analysis and gene ontology, gene expression, differential expression, response to biotic and abiotic stresses, correlation with productivity and yield. Proteome analysis, differential protein synthesis, protein interactions, post-translational modifications, proteome modulation during plant growth and development, relationships with stress response, network mapping. Metabolome analysis, metabolic responses to stresses, secondary metabolism and medicinal and aromatic plants, characterization of metabolites in genetically modified plants. Analysis of metabolic fluxes. Detection of plant diseases by molecular techniques. Application of biodiagnostic technologies in the identification of species and varieties.
Keywords
genomics, metagenomics, transcriptomics, proteomics, metabolomics, bioinformatics, next generation sequencing
Description
Written Examination with Multiple Choice Questions (Formative, Inferential) Written Examination with Short Answer Questions (Formative, Inferential) Written Examination with Extended Answer Questions (Formative, Inferential) Written Assignment (Formative, Inferential) Public Presentation (Formative)
Evaluation Methods:
Written Exam with Multiple Choice Questions (Formative, Inferential), Written Exam with Short Answer Questions (Formative, Inferential), Written Exam with Extended Answer Questions (Formative, Inferential), Written Assignment (Formative, Inferential), Public Presentation (Formative)
Additional bibliography for study
Plant Cell Physiol. 52(12): 2017–2038 (2011) doi:10.1093/pcp/pcr153
J. Agric. Food Chem. 2006, 54, 8984-8994 , https://pubs.acs.org/doi/10.1021/jf061218t
Kumar R, Bohra A, Pandey AK, Pandey MK and Kumar A (2017) Metabolomics for Plant Improvement: Status and Prospects. Front. Plant Sci. 8:1302. doi: 10.3389/fpls.2017.01302