Operation of a Medical Microbiology Laboratory, Good Laboratory Practice, Biosafety
| Title | Λειτουργία εργαστηρίου Ιατρικής Μικροβιολογίας, Καλή Εργαστηριακή πρακτική, Βιοασφάλεια / Operation of a Medical Microbiology Laboratory, Good Laboratory Practice, Biosafety |
| Code | ΜΙΜΙΚΑ001 |
| Faculty | Health Sciences |
| School | Medicine |
| Cycle / Level | 2nd / Postgraduate |
| Teaching Period | Winter |
| Coordinator | Melania Kachrimanidou |
| Common | No |
| Status | Active |
| Course ID | 600021719 |
Programme of Study: PMS "Sýnchronī Efarmosménī Mikroviología" (2022-sīmera)
Registered students: 28
| Orientation | Attendance Type | Semester | Year | ECTS |
|---|---|---|---|---|
| KORMOS | Compulsory Course | 1 | 1 | 6 |
| Academic Year | 2024 – 2025 |
| Class Period | Winter |
| Faculty Instructors | |
| Instructors from Other Categories |
|
| Weekly Hours | 4 |
| Total Hours | 52 |
| Class ID | 600261808
|
Course Type 2021
Specific Foundation
Mode of Delivery
- Face to face
- Distance learning
Digital Course Content
- e-Study Guide https://qa.auth.gr/en/class/1/600261808
- eLearning: https://elearning.auth.gr/course/view.php?id=17996
Language of Instruction
- Greek (Instruction, Examination)
- English (Instruction)
General Competences
- Apply knowledge in practice
- Retrieve, analyse and synthesise data and information, with the use of necessary technologies
- Work autonomously
- Work in teams
- Generate new research ideas
- Advance free, creative and causative thinking
Educational Material Types
- Notes
- Slide presentations
- Video lectures
Use of Information and Communication Technologies
Use of ICT
- Use of ICT in Course Teaching
- Use of ICT in Communication with Students
- Use of ICT in Student Assessment
Course Organization
| Activities | Workload | ECTS | Individual | Teamwork | Erasmus |
|---|---|---|---|---|---|
| Lectures | 26 | 1.0 | |||
| Reading Assigment | 28 | 1.1 | |||
| Tutorial | 43 | 1.7 | |||
| Exams | 1 | 0.0 | |||
| Other / Others | 52 | 2.1 | |||
| Total | 150 | 6 |
Student Assessment
Student Assessment methods
- Written Exam with Multiple Choice Questions (Formative, Summative)
- Oral Exams (Formative, Summative)
Bibliography
Additional bibliography for study
1. Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 7th ed, 2017 (chapter 1, 3, 4)
2. Textbook of Diagnostic Microbiology, Mahon, Lehman, Manuselis, 5th ed, 2015 (Part I, chapter 6, 7, 10, 11)
3. Manual of Clinical Microbiology, Jorgensen J., Pfaller M., 11th ed, 2015, (Vol 1, section 1, chapter 2, 6, 7)
4. Jawetz, Melnick & Adelberg’s Medical Microbiology by Riedel, Morse, Mietzner, Miller, 28th ed, 2019 (VII, chapter 47)
5. Murray P, Rosenthal K, Kobayashi K, Pfaller M. Medical Microbiology. 7th ed, 2012, Elsevier.
6. Warren Levinson. Review of Medical Microbiology and Immunology, 13th ed, 2014, Lange Medical Books.
7. Tortora G, Funke B, Case S, Επιμέλεια Α. Τσακρής. Εισαγωγή Στην Ιατρική Μικροβιολογία, 12th ed, 2016, Broken Hill.
8. Bailey and Scott’s. Diagnostic Microbiology. 11th ed, 2002, Mosby.
9. Al-Omari MM, Qaqish A, Omeish HAF, Najadat AAA, Mahasneh F, Al-Tamimi M, Abbas MM, Al-Qaoud K. Review of current in vitro COVID-19 diagnostics methods. J Infect Dev Ctries. 2022 Sep 30;16(9):1376-1384. doi: 10.3855/jidc.16378. PMID: 36223610.
10. Kaufman Α, Brewster R, Rajasekaran K, How to Perform a Nasopharyngeal Swab − An Otolaryngology Perspective. The American Journal of Medicine, Department of Otorhinolaryngology − Head and Neck Surgery, University of Pennsylvania. 2020. doi: https://doi.org/10.1016/j.amjmed.2020.05.004
11. Kwon YJ, Kwak HJ, Lee HK, Lim HC, Jung DH. Comparison of bacterial community profiles from large intestine specimens, rectal swabs, and stool samples. Appl Microbiol Biotechnol. 2021 Dec; 105(24): 9273-9284. doi: 10.1007/s00253-021-11650-y. Epub 2021 Nov 13. PMID: 34773153.
12. Lu H, Ma L, Zhang H, Feng L, Yu Y, Zhao Y, Li L, Zhou Y, Song L, Li W, Zhao J, Liu L. The Comparison of Metagenomic Next-Generation Sequencing with Conventional Microbiological Tests for Identification of Pathogens and Antibiotic Resistance Genes in Infectious Diseases. Infect Drug Resist. 2022 Oct 22; 15:6115-6128. doi: 10.2147/IDR.S370964. PMID: 36277249; PMCID: PMC9586124.
13. Laura L Hammitt, David R Murdoch, J Anthony G Scott, Amanda Driscoll, Ruth A Karron, Orin S Levine, Katherine L O'Brien; Pneumonia Methods Working Group. Specimen collection for the diagnosis of pediatric pneumonia. Clin Infect Dis 2012 Apr;54 Suppl 2 (Suppl 2): S132-9, doi: 10.1093/cid/cir1068.
14. Turner G, O'Grady M, Hudson D, Morgan X, Frizelle F, Purcell R. Rectal swabs are a reliable method of assessing the colonic microbiome. Int J Med Microbiol. 2022 Feb; 312(2):151549. doi: 10.1016/j.ijmm.2022.151549. Epub 2022 Jan 24. PMID: 35114582.
15. Waagsbø B, Buset EM, Longva JÅ, Bjerke M, Bakkene B, Ertesvåg AS, Holmen H, Nikodojevic M, Tran TT, Christensen A, Nilsen E, Damås JK, Heggelund L. Diagnostic stewardship aiming at expectorated or induced sputum promotes microbial diagnosis in community-acquired pneumonia. BMC Infect Dis. 2022 Mar 2; 22(1): 203. doi: 10.1186/s12879-022-07199-4. PMID: 35236305; PMCID: PMC8889388.
16. Wang Y, Hu X, Yang L, Chen C, Cheng H, Hu H, Liang W, Tong Y, Wang M, Wang H. Application of High-Throughput Sequencing Technology in the Pathogen Identification of Diverse Infectious Diseases in Nephrology Departments. Diagnostics (Basel). 2022 Sep 1; 12(9): 2128. doi: 10.3390/diagnostics12092128. PMID: 36140529; PMCID: PMC9497786.
17. Yang H, Smith RD, Sumner KP, Goodlett DR, Johnson JK, Ernst RK. A Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Direct-from-Urine-Specimen Diagnostic for Gram-Negative Pathogens. Microbiol Spectr. 2022 Oct 18: e0373022. doi: 10.1128/spectrum.03730-22. Epub ahead of print. PMID: 36255333.
18. Αρσένη Α. Κλινική Μικροβιολογία και Εργαστηριακή Διάγνωση Λοιμώξεων, 1994, Εκδόσεις Ζήτα.
19. WMA Declaration of Helsinki- Ethical Principles for Medical research involving Human subjects.
20. Nόμος 2619/1998 (ΦΕΚ Α΄132) Σύμβαση του Οβιέδο
21. Global Health Ethics. Global network of WHO collaborating centers for Bioethics, 2015
22. Eθνική Επιτροπή Βιοηθικής. Κώδικας δεοντολογίας για την έρευνα στις Βιολογικές Επιστήμες, Οκτώβριος 2008.
23. Koneman’s Color Atlas and Textbook of Diagnostic Microbiology, Seventh Edition, 2017 Wolters Kluwer: Chapter 1: Introduction to Microbiology Part I: The role of the Microbiology Laboratory in the Diagnosis of Infectious Diseases: Guidelines to Practice and Management, p: 45-65, Chapter 19: Mycobacteria, p:1219-1268, Chapter 21: Mycology, p: 1322-1416
24. Ιατρική Μικροβιολογία Murray, Rosenthal, Pfaller, 8η έκδοση (3η ελληνική έκδοση, 2016 Επιστημονικές Εκδόσεις Παρισιάνου): Κεφάλαιο 22: Mycobacterium (Μυκοβακτηρίδιο) και συγγενή οξεάντοχα βακτήρια, σ. 253-270, Κεφάλαιο 29: Βρουκέλλα, σ: 339-341, Κεφάλαιο 31: Μη σπορογόνα αναερόβια βακτήρια, σ. 363-375, Κεφάλαιο 60: Εργαστηριακή διάγνωση νόσων από μύκητες, σ. 674-684.
25. The clinical Laboratory (Part 1) in: Ηenry’s Clinical Diagnosis and Management by Laboratory Methods, 24th edition, Elsevier, Philadelphia 2022.
26. Aθανάσιος Βυζαντιάδης. Ποιοτικός έλεγχος - Σφάλματα μετρήσεων. Μικροβιολογικά Χρονικά 1987, 3: 14-36.
27. Εkins Roger. Immunoassay standardization. Scand J Clin Lab Invest Suppl. 1991; 205: 33-46.
28. T.-A. Βυζαντιάδης. Πρότυπα μετρήσεων-γενικές γνώσεις για τα αντιδραστήρια και την εργαστηριακή μεθοδολογία. Σημειώσεις στα πλαίσια του μεταπτυχιακού μαθήματος κορμού Β΄ εξαμήνου: Κλινικο-εργαστηριακή μεθοδολογία. ΠΜΣΙ Ιατρική Ερευνητική Μεθοδολογία, Ιατρική Σχολή ΑΠΘ, 2004-2012.
29. Bermes EW, Young DS. General Laboratory Techniques and Procedures.In: Tietz NW. Fundamentals of Clinical Chemistry. Third Edition. W. B. Saunders Company 1987.
30. Αρσένη Α. Δεληγιάννη Β, Ζουλιέν Ζ. Ιατρική Βιοχημεία. 2η Έκδοση. Αθήνα: Ιατρικές Εκδόσεις «ΖΗΤΑ» 1991.
31. Γαλανοπούλου-Γκιουζέπα Β. Μέθοδοι αποστείρωσης. Μικροβιολογικά Χρονικά 1995, 11: 266-278.
32. Καραμούζης Μ. Χημικά διαλύματα στην εργαστηριακή πράξη. Μικροβιολογικά Χρονικά 1994, 10: 302-312.
33. What are the key activities in a Microbiology laboratory? by Ankit Khurana, 2015. In: Lab- training.com.
34. Pence MA, Liesman R. Clinical Microbiology. Chapter 55: in Contemporary Practice in Clinical Chemistry. DOI: https://doi.org/10.1016/B978-0-12-815499-1.00055-7, 2020 Elsevier Inc.
35. Biosafety in microbiological and biomedical laboratories, June 2020, By Meechan, Paul J. ; Potts, Jeffrey, Series: HHS publication ; no. (CDC) 300859
36. https://www.who.int/publications/i/item/9789241516266
37. Biological safety cabinets and other primary containment devices. Geneva: World Health Organization; 2020(Laboratory biosafety manual, fourth edition and associated monographs). Licence: CC BY-NC-SA 3.0 IGO.
38. Laboratory biosafety manual, fourth edition. Geneva: World Health Organization; 2020 (Laboratory biosafety manual, fourth edition and associated monographs. Licence: CC BY-NC-SA 3.0 IGO.
39. ΦΕΚ244_Α_7-12-2020_
40. WHO guidance on implementing regulatory requirements for biosafety and biosecurity in biomedical laboratories – a stepwise approach. Geneva: World Health Organization; 2020. Licence: CC BY-NC-SA 3.0 IGO
41. Steering Committee on Bioethics - Council of Europe, rm.coe.int › 1680307ebf
42. WMA DECLARATION OF HELSINKI – ETHICAL PRINCIPLES FOR MEDICAL RESEARCH INVOLVING HUMAN SUBJECTS,https://www.wma.net/policies-post/wma-declaration-of-helsinki-ethical-principles-for-medical-research-involving-human-subjects/
43. International Ethical Guidelines for Biomedical Research Involving Human Subjects Prepared by the Council for International Organizations of Medical Sciences(CIOMS) in collaboration with the World Health Organization(WHO)2002
44. Νόµος 2619/1998 (ΦΕΚ Α'132,Κύρωση της Σύµβασης του Συµβουλίου της Ευρώπης για την προστασία των
ανθρωπίνων δικαιωµάτων και της αξιοπρέπειας του ατόµου σε σχέση µε τις εφαρµογές της βιολογίας και της ιατρικής: Σύµβαση για τα Ανθρώπινα ∆ικαιώµατα και τη Βιοϊατρική
45. https://www.who.int/publications/i/item/global-health-ethics-key-issues
46. WMA INTERNATIONAL CODE OF MEDICAL ETHICS
47. Υπόδειγµα Κώδικα ∆εοντολογίας Για την έρευνα στις βιολογικές επιστήµες, ΕΘΝΙΚΗ ΕΠΙΤΡΟΠΗ ΒΙΟΗΘΙΚΗΣ,Οκτώβριος 2008
Last Update
16-01-2024
