Research Methodology - Laboratory Techniques in Periodontology
| Title | Ερευνητική Μεθοδολογία - Εργαστηριακές Τεχνικές / Research Methodology - Laboratory Techniques in Periodontology |
| Code | S0307 |
| Faculty | Health Sciences |
| School | Dentistry |
| Cycle / Level | 2nd / Postgraduate |
| Teaching Period | Winter/Spring |
| Coordinator | Sotirios Kalfas |
| Common | No |
| Status | Active |
| Course ID | 600023077 |
Programme of Study: PMS Periodontología kai Viología Emfyteumátōn (2024-sīmera)
Registered students: 4
| Orientation | Attendance Type | Semester | Year | ECTS |
|---|---|---|---|---|
| PERIODONTOLOGIA KAI VIOLOGIA EMFYTEUMATŌN | Compulsory Course | 3 | 2 | 2 |
| Academic Year | 2025 – 2026 |
| Class Period | Winter |
| Faculty Instructors | |
| Class ID | 600287209
|
Course Type 2021
Specific Foundation
Mode of Delivery
- Face to face
Digital Course Content
- e-Study Guide https://qa.auth.gr/en/class/1/600287209
- At the Website of the School: Υπό κατασκευή
Language of Instruction
- Greek (Instruction, Examination)
- English (Instruction, Examination)
Prerequisites
General Prerequisites
Ν/Α
Learning Outcomes
To acquire knowledge in laboratory methods used in periodontal research and search-engines in databases.
General Competences
- Apply knowledge in practice
- Retrieve, analyse and synthesise data and information, with the use of necessary technologies
- Adapt to new situations
- Be critical and self-critical
- Advance free, creative and causative thinking
Course Content (Syllabus)
Research protocol design Ι
Research protocol design ΙI
Database search methodologies
Database search methodologies
Database search methodologies
Systematic research (secondary
clinical research)
Clinical intervention studies - design
Failures in dental research –
"research waste"
Laboratory techniques - Cultivation techniques – aerobic, anaerobic
Laboratory techniques - ELISA / PCR / Deep sequencing of the microbiome
Laboratory techniques - Chair-side microbial screening
Proteomics in periodontal research I
Proteomics in periodontal research II
Final Evaluation
Keywords
Research methodology, Laboratory techiques
Educational Material Types
- Notes
- Slide presentations
- Book
Use of Information and Communication Technologies
Use of ICT
- Use of ICT in Course Teaching
- Use of ICT in Laboratory Teaching
- Use of ICT in Communication with Students
Description
As above
Course Organization
| Activities | Workload | ECTS | Individual | Teamwork | Erasmus |
|---|---|---|---|---|---|
| Lectures | 26 | ||||
| Seminars | 26 | ||||
| Tutorial | 0 | ||||
| Total | 52 |
Student Assessment
Description
Performance during the seminars and at the final exam
Student Assessment methods
- Written Exam with Short Answer Questions (Summative)
- Written Assignment (Summative)
Bibliography
Additional bibliography for study
Research protocol design
Based on guidelines: https://www.who.int/groups/research-ethics-review-committee/recommended-format-for-a-research-protocol/
Cameli M, Novo G, Tusa M, Mandoli GE, Corrado G, Benedetto F, Antonini-Canterin F, Citro R. How to Write a Research Protocol: Tips and Tricks. J Cardiovasc Echogr. 2018 Jul-Sep;28(3):151-153. doi: 10.4103/jcecho.jcecho_41_18. PMID: 30306017; PMCID: PMC6172884.
Database search methodologies
The courses of this section are being taught in the main library of the Aristotle University of Thessaloniki, where students are trained in searching databases by the librarians.
Failures in dental research - "Research Waste"
1. Pandis N, Fleming PS, Katsaros C, Ioannidis JPA. Dental Research Waste in Design, Analysis, and Reporting: A Scoping Review. J Dent Res. 2021 Mar;100(3):245-252. doi: 10.1177/0022034520962751. Epub 2020 Oct 15. PMID: 33054504.
2. Faggion CM Jr, Listl S, Smits KPJ. Meta-research publications in dentistry: a review. Eur J Oral Sci. 2021 Feb;129(1):e12748. doi: 10.1111/eos.12748. Epub 2021 Feb 3. PMID: 33533130.
3. Gussy M, Dickson-Swift V, Adams J. A scoping review of qualitative research in peer-reviewed dental publications. Int J Dent Hyg. 2013 Aug;11(3):174-9. doi: 10.1111/idh.12008. Epub 2012 Dec 13. PMID: 23241429.
4. Layton DM, Clarke M. Research Waste: How Are Dental Survival Articles Indexed and Reported? Int J Oral Maxillofac Implants. 2016 Jan-Feb;31(1):125-32. doi: 10.11607/jomi.4590. PMID: 26800169.
5. Blanco D, Kirkham JJ, Altman DG, Moher D, Boutron I, Cobo E. Interventions to improve adherence to reporting guidelines in health research: a scoping review protocol. BMJ Open. 2017 Nov 16;7(11):e017551. doi: 10.1136/bmjopen-2017-017551. PMID: 29150467; PMCID: PMC5701974.
6. Turner L, Shamseer L, Altman DG, Weeks L, Peters J, Kober T, Dias S, Schulz KF, Plint AC, Moher D. Consolidated standards of reporting trials (CONSORT) and the completeness of reporting of randomised controlled trials (RCTs) published in medical journals. Cochrane Database Syst Rev. 2012 Nov 14;11(11):MR000030. doi: 10.1002/14651858.MR000030.pub2. PMID: 23152285; PMCID: PMC7386818.
Sytematic review research
https://training.cochrane.org/handbook/current/part-2
Laboratory culture techniques - Aerobic and anaerobic - ELISA / PCR - DNA-DNA checkerboard - Chair-side microbial screening - Deep sequencing of the microbiome - Proteomics in periodontal research I,II
1. Sakellari D, Arapostathis KN, Konstantinidis A. Periodontal conditions and subgingival microflora in Down syndrome patients. A case-control study. J Clin Periodontol. 2005 Jun;32(6):684-90. doi: 10.1111/j.1600-051X.2005.00737.x. PMID: 15882231.
2. Sakellari D, Belibasakis G, Chadjipadelis T, Arapostathis K, Konstantinidis A. Supragingival and subgingival microbiota of adult patients with Down's syndrome. Changes after periodontal treatment. Oral Microbiol Immunol. 2001 Dec;16(6):376-82. doi: 10.1034/j.1399-302x.2001.160610.x. PMID: 11737662.
3. Xajigeorgiou C, Sakellari D, Slini T, Baka A, Konstantinidis A. Clinical and microbiological effects of different antimicrobials on generalized aggressive periodontitis. J Clin Periodontol. 2006 Apr;33(4):254-64. doi: 10.1111/j.1600-051X.2006.00905.x. PMID: 16553634.
4. Baumgartner D, Johannsen B, Specht M, Lüddecke J, Rombach M, Hin S, Paust N, von Stetten F, Zengerle R, Herz C, Peham JR, Paqué PN, Attin T, Jenzer JS, Körner P, Schmidlin PR, Thurnheer T, Wegehaupt FJ, Kaman WE, Stubbs A, Hays JP, Rusu V, Michie A, Binsl T, Stejskal D, Karpíšek M, Bao K, Bostanci N, Belibasakis GN, Mitsakakis K. OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice. Biosensors (Basel). 2021 Oct 28;11(11):423. doi: 10.3390/bios11110423. PMID: 34821641; PMCID: PMC8615610.
5. Bostanci N, Grant M, Bao K, Silbereisen A, Hetrodt F, Manoil D, Belibasakis GN. Metaproteome and metabolome of oral microbial communities. Periodontol 2000. 2021 Feb;85(1):46-81. doi: 10.1111/prd.12351. Epub 2020 Nov 23. PMID: 33226703.
6. Bostanci N. Revisiting "-omics" in Oral Health and Disease. Proteomics Clin Appl. 2020 May;14(3):e1900022. doi: 10.1002/prca.201900022. PMID: 32426939.
7. Johansson A, Claesson R, Hänström L, Sandström G, Kalfas S. Polymorphonuclear leukocyte degranulation induced by leukotoxin from Actinobacillus actinomycetemcomitans. J Periodontal Res. 2000 Apr;35(2):85-92. doi: 10.1034/j.1600-0765.2000.035002085.x. PMID: 10863962.
8. Holm A, Rabe P, Kalfas S, Edwardsson S. Improved selective culture media for Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus. J Clin Microbiol. 1987 Oct;25(10):1985-8. doi: 10.1128/jcm.25.10.1985-1988.1987. PMID: 3667919; PMCID: PMC269382.
9. Apatzidou, Danae Anastasia (2001) Clinical, Microbiological and Immunological Responses to Two Non-Surgical Periodontal Treatment Modalities. PhD thesis, University of Glasgow.
10. Ganesan SM, Dabdoub SM, Nagaraja HN, Mariotti AJ, Ludden CW, Kumar PS. Biome-microbiome interactions in peri-implantitis: a pilot investigation. J Periodontol. 2022 Jan 24. doi: 10.1002/JPER.21-0423. Epub ahead of print. PMID: 35073418.
11. Kumar PS. Microbiomics: Were we all wrong before? Periodontol 2000. 2021 Feb;85(1):8-11. doi: 10.1111/prd.12373. Epub 2020 Nov 23. PMID: 33226670.
12. Mason MR, Nagaraja HN, Camerlengo T, Joshi V, Kumar PS. Deep sequencing identifies ethnicity-specific bacterial signatures in the oral microbiome. PLoS One. 2013 Oct 23;8(10):e77287. doi: 10.1371/journal.pone.0077287. Erratum in: PLoS One. 2014;9(6):e99933. PMID: 24194878; PMCID: PMC3806732.
13. Apatzidou D, Lappin DF, Hamilton G, Papadopoulos CA, Konstantinidis A, Riggio MP. Microbiome of peri-implantitis affected and healthy dental sites in patients with a history of chronic periodontitis. Arch Oral Biol. 2017 Nov;83:145-152. doi: 10.1016/j.archoralbio.2017.07.007. Epub 2017 Jul 13. PMID: 28780383.
Last Update
10-01-2024
