Learning Outcomes
At the end of the course, students will be able to:
1) Deeply understand the laws of inheritance, the Cytogenetics and the
Εvolution of Crop plants.
2) Plan and apply methods for genome analysis, caryotyping and gene mapping.
3) Understand deeply the Cell cycle and the role of Mitosis and Meiosis in
plant development, reproduction and diversity
4) Learn the type of mutations and their role in genetic variation and use
of articifial Mutation methods for the development of useful genetic
variability
5) Know and apply all the approaches and techniques for ploidy determination,
haploid production and hybridization for transfer useful genes from a wild
relative to a cultivated variety.
6) Be Familiar with the new technologies of Molecular Plant Cytogenetics
Course Content (Syllabus)
SYLLABUS
The inheritance of Genetic Material
How the Eukariotic Genome is organized.
Structure and type of Chromosomes.
Mitochodrial and Chloroplast DNA.
Cell Cycle (G1, S, G2, M)
MITOSIS and MEIOSIS - Biological Meaning for Organisms
C- Value
Ploidy Level
Karyotype Analysis (Theory and Lab)
Flow Cytometry Analysis (Theory and Lab)
Linkage of genes and Crhomosome Mapping
Types of Genetic Maps and DNA Sequencing
Study of Mutations and their role in Genetic diversity and evolution
Artificial Mutations in Plant Cytogenetics
The role of Haploids and polyploids in Plant Breeding
Techniques in Haploid and Polyploid Production
Genome analysis in Aneuplois and Polyploids
Phenomena of Apomixis, Parthenogenesis, Male sterility ans Self incompatipility
In Vitro techniques in Plant Genetics & Breeding
Hybridization in Plant species - Role and Techniques
Inter and Intra - specific hybridization
Transfer of genes from wild relatives to Crop species
Modern techniques of Molecular Cytogenetics -In situ Hybridization-FISH-GISH-GENE EDITING
Applications of Cytogenetics in Plant Breeding Programs
Keywords
Cytogenetics, chromosomes, Gene Mapping, Mutations, Interspecific Hybridization, Gene Editing
Additional bibliography for study
1. I – GENETICS
2. Plant Gytogenetics (R. Singh) - Chapter 4
3. Cytogenetics (J. Schulz – Schaeffer) – Chapters 2, 3, 4
European guidelines for constitutional cytogenomic analysis
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