Laboratory Molecular Biology in Exercise

Skills Development

The course is also offered to exchange programme students.

Upon successful completion of the course, students will be able to - recognize the basic equipment of a Molecular Biology Laboratory - understand and follow a laboratory protocol - explain the theoretical background of some of the basic Molecular Biology techniques (such as ELISA, PCR) - comprehend the scientific application of Molecular Biology techniques in Sports and health in general Learning Outcomes Upon successful completion of this course, students will acquire: Knowledge: • Understanding the theory behind fundamental molecular biology techniques, such as PCR and ELISA. • Familiarity with the functions and usage of basic equipment used in a Molecular Biology laboratory. • Insight into the relationship between molecular techniques and the study of phenomena related to exercise and health. Skills: • Ability to perform basic molecular biology experiments with precision and safety. • Competence in reading, understanding, and applying laboratory protocols. • Proper use of essential laboratory tools, such as micropipettes and centrifuges. Competencies: • Development of scientific thinking and a systematic approach to solving laboratory problems. • Connection of theoretical knowledge with practical applications in the field of exercise and health. • Collaboration in the laboratory, adhering to safety rules and teamwork principles.

-General Introduction -“Virtual tour” to a Molecular Biology Laboratory -Introduction to Microscopy -Morphological differentiation of leucocytes, blood types and haematocrit -Diffusion-Osmosis -Spectrophotometry-pH -Making Solutions in the Lab -DNA Miniprep (DNA extraction) -ELISA -PCR -Factors that affect Heart rate -Enzyme reactions -Molecular Biology Quiz Week 1 Introduction to course structure, objectives, and assessment methods. Discussion on basic principles of Molecular Biology and its significance in health and exercise. • Understand the course's purpose and structure. - Familiarize with the field of Molecular Biology and its applications. Week 2 Laboratory tour and familiarization with equipment. Presentation and execution of a simple experimental protocol. • Identify basic laboratory equipment. - Understand the structure and usage of an experimental protocol. Week 3 Theoretical and practical approach to microscopy. Observation of cellular structures (blood cells) using a light microscope. • Use a microscope to observe biological samples. - Comprehend the importance of microscopy in Molecular Biology. Week 4 Interpretation of Complete Blood Count results – Blood groups – Hematocrit – Leukocyte differential count. • Interpret basic hematological parameters. - Understand the diagnostic value of blood tests. Week 5 Demonstration experiments simulating physical phenomena of diffusion and osmosis. • Grasp the basic principles of diffusion and osmosis. - Relate to cellular function and homeostasis. Week 6 Measurement and regulation of pH. • Use a pH meter. - Understand the significance of pH in biological systems. Week 7 Preparation of various solutions (Concentrations – Dilutions). • Calculate concentrations and prepare solutions. - Acquaintance with dilutions and basic laboratory techniques. Week 8 DNA Isolation. • Perform DNA isolation procedures. - Understand the significance of DNA for molecular analysis. Week 9 Theoretical approach to ELISA methodology and immunofluorescence techniques. • Understand the operation and use of ELISA and immunofluorescence techniques. - Relate to protein and pathogen detection. Week 10 Theory behind molecular detection tests – PCR. • Understand the process and significance of PCR. - Analyze its applications in medicine and biology. Week 11 Effect of various stimuli on the heart (study of heart rate). • Record and analyze heart rate. - Comprehend the physiological response of the heart to stimuli. Week 12 Study of Enzymatic Reactions through experiments. • Understand the parameters affecting enzyme activity. Week 13 Laboratory Molecular Biology Quiz. • Team activity – review quiz. - Reinforce acquired knowledge. - Develop collaborative and creative spirit.

molecular biology in exercise

17-06-2025