Molecular Biotechnology
| Title | Μοριακή Βιοτεχνολογία / Molecular Biotechnology |
| Code | M1Y33 |
| Faculty | Sciences |
| School | Chemistry |
| Cycle / Level | 2nd / Postgraduate |
| Teaching Period | Spring |
| Common | No |
| Status | Active |
| Course ID | 600015675 |
Programme of Study: Synthetic Chemistry, Biochemistry and Applications
Registered students: 4
| Orientation | Attendance Type | Semester | Year | ECTS |
|---|---|---|---|---|
| "Anórganes Enṓseis, Yliká kai Efarmogés" | Elective Courses | 2 | 1 | 10 |
| "Organikī Sýnthesī kai Efarmogés" | Elective Courses | 2 | 1 | 10 |
| "Viochīmeía" | Compulsory Course | 2 | 1 | 10 |
| Academic Year | 2024 – 2025 |
| Class Period | Spring |
| Faculty Instructors | |
| Weekly Hours | 3 |
| Class ID | 600266762
|
Mode of Delivery
- Face to face
Digital Course Content
- e-Study Guide https://qa.auth.gr/en/class/1/600266762
Language of Instruction
- Greek (Instruction, Examination)
Learning Outcomes
Analyze and apply gene cloning techniques, such as reverse transcription and PCR, for the production and amplification of genetic material.
Select and utilize appropriate expression vectors for gene transfer and expression in prokaryotic and eukaryotic systems.
Understand and apply methods of eukaryotic cell transfection for experimental or therapeutic purposes.
Evaluate the use of nanoparticles, dendrimers, and liposomes as innovative therapeutic delivery systems at the molecular and cellular level.
Interpret the function and applications of various types of biosensors, such as enzymatic and DNA-based biosensors.
Apply plasmid DNA isolation techniques and understand the biotechnological applications of triple-helix DNA structures.
Understand the structure, mechanism of action, and applications of biosurfactants in environmental and industrial contexts.
General Competences
- Apply knowledge in practice
- Retrieve, analyse and synthesise data and information, with the use of necessary technologies
- Work autonomously
- Work in teams
- Generate new research ideas
- Be critical and self-critical
Course Content (Syllabus)
Gene cloning using reverse transcriptase and polymerase chain reaction (PCR). Synthesis of DNA (a gene or gene fragment) from RNA via reverse transcription. Amplification of DNA fragments using PCR. Cloning into suitable expression vectors. Gene expression in prokaryotic and eukaryotic cells. Methods for transfection of eukaryotic cells.
Nanoparticles, dendrimers, and liposomes as therapeutic nanocarriers for enzyme inhibition and disease treatment.
Biosensors: enzymatic biosensors and DNA biosensors. Plasmid DNA isolation and biotechnological applications based on triple-helix DNA structures.
Biosurfactants: structure, function, and applications.
Keywords
PCR techniques, nanoparticles, bioreceptors
Educational Material Types
- Notes
- Slide presentations
Use of Information and Communication Technologies
Use of ICT
- Use of ICT in Course Teaching
- Use of ICT in Communication with Students
Course Organization
| Activities | Workload | ECTS | Individual | Teamwork | Erasmus |
|---|---|---|---|---|---|
| Lectures | 18 | ||||
| Seminars | 9 | ||||
| Total | 27 |
Student Assessment
Student Assessment methods
- Written Exam with Extended Answer Questions (Formative, Summative)
- Performance / Staging (Formative, Summative)
Last Update
07-07-2025
