Learning Outcomes
The students will widen further their knowledge of Biotechnology, in particular the role of microorganisms, genetic engineering, enzymes and bioseparations. This knowledge is considered necessary for a Chemical Engineer, since the application of biotechnology is often required both in traditional chemical engineering and in modern biochemical engineering and biomedical applications.
Course Content (Syllabus)
Historical overview. Cell: Procaryotic and eukaryotic cells. Morphology. Chemical components of cells. Microorganisms. Important cell types. Cell cultures (animal, plant and microbial). Kinetics of cell growth-Equations. Methods for determination of cell growth. Enzymes. Kinetics of enzyme-catalyzed reactions. Michaelis-Menten equation. Briggs-Haldone kinetics. Evaluations of parameters in the Michaelis-Menten equation. Effect of pH and tempwrature on Enzyme kinetics. Order of enzyme-catalyzed reaction. Inhibition. Reversible inhibition. Competitive and non competitive inhibition. Immobilized enzymes and applications: Methods for immobilization. Examples. Applications of immobilized enzymes in industry. Production of L-amino acids with immobilized. a-amino acylase, β-galactosidase for hydrolysis of lactose, semi synthetic penicillins. Commercial immobilized enzymes. Sterilization: Methods of sterilization. Heat, chemical reagents, mechanical methods, ultrasound. Sterilization by heating. Continuous and non continuous sterilization. Bioreactors: Types of bioreactors. Productivity and designing of bioreactors. Factors influencing the operation of a bioreactor. Bioseparations-Dowbstream processing: General. Broth handling Seperation of liquid-solid. Filtration, Centrifugation. Cell lysis (Chemical, Enzymic and Physical methods). Product recaovery: Extraction. Adsorption. Product purification: Precipitation, Electrophoresis, Chromatography ion exchange, affinity and gell permeation chromatography). Ultrafiltration. Biotechnological applications. Synthesis and modification of polymers. Proteins and polypeptides Oligo- and polysaccharides. Degradation of starch by enzymes. Single cell proteins. Biosensors of proteins.
Keywords
Microorganisms, Immobilized enzymes, Bioseparations-Dowbstream processing, Biotechnological applications
Additional bibliography for study
1. Biochemical Engineering and Biotechnology
Ghasem Najafpour
Elsevier Science 2006
2. Future Trends in Biotechnology
Chikara Furusawa, Takaaki Horinouchi, Takashi Hirasawa (auth.), Jian-Jiang Zhong
(eds.)
Advances in Biochemical Engineering/Biotechnology 131
Springer-Verlag Berlin Heidelberg 2013