Research Scientist 1993 - 1995
Massachusetts Institute of Technology, Cambridge,
Massachusetts, USA
Comparative investigation of the effects of inorganic nutrients in the growth of the wild type methanotrophic
bacteria Methylococcus capsulatus and Methylosinus trichosporium. Development of large scale growth
of the wild type isotopically enriched (57Fe, 54Fe) methanotrophic bacterium Methylococcus capsulatus.
Investigation of biological activity, chemical and structural properties of substitutionally derivatized
hydroxylase enzyme in Methylococcus capsulatus. Cloning and expression of methanotrophic bacterial
genes in E. coli.
Special Scientific Staff 1993
New England Medical Center/Tufts University, Boston,
Massachusetts, USA.
Investigation of the functional capabilities of Hα-RAS gene minisatellites in human cell extracts. Instability
of telomeric gene minisatellites and interactions with transcription factors in carcinogenesis. Phylogeny and functional implications of recombinatorial chi (χ) sequence signals in genes across the evolutionary spectrum of species.
Postdoctoral Associate 1990 - 1992
Massachusetts Institute of Technology, Cambridge,
Massachusetts, USA
Development of high yield, high activity biochemical techniques for isolation and purification of the
metalloenzyme enzyme methane monooxygenase (MMO). Development of protocols for large scale fermentations of the methanotrophic bacteria Methylococcus capsulatus and Methylosinus trichosporium. Cloning, DNA sequencing and expression of the alpha subunit of the MMO hydroxylase protein in E. coli. Design and development of large scale growth for recombinant E. coli organisms.
NATO Postdoctoral Research Associate 1988 - 1990
Massachusetts Institute of Technology, Cambridge,
Massachusetts, USA
New methods of high yield extraction, purification and spectroscopic/structural characterization of the cofactor
of the molybdenum nitrogenase enzyme. Development of growth medium and protocols for high yield pilot plant fermentations of the nitrogen fixing bacterium Azotobacter vinelandii. Isolation, purification and characterization of the metalloenzyme nitrogenase and its protein components.
Research Assistant 1983 - 1987
The University of Michigan, Ann Arbor,
Michigan, USA
Design of stoichiometric and structural models of metal containing active sites of proteins and enzymes involved
in biological electron transfer and catalysis. Synthesis, reactivity investigation, spectroscopic and X-ray structural characterization of inorganic heterometallic clusters as potential models for the active sites of metalloenzymes.
Research Trainee Summer 1981
Max-Planck Institute for Radiation Chemistry,
Mülheim an der Ruhr, Germany
Mechanistic studies and reactivity exploration of organic compounds using γ-irradiation techniques.
