Laboratory techniques in Biochemistry

Course Information
TitleΕργαστηριακές Τεχνικές στη Βιοχημεία / Laboratory techniques in Biochemistry
CodeΜΧ033
FacultySciences
SchoolChemistry
Cycle / Level2nd / Postgraduate
Teaching PeriodWinter
CommonNo
StatusActive
Course ID40003161

Programme of Study: PPS of School of Chemistry (2014-2015)

Registered students: 4
OrientationAttendance TypeSemesterYearECTS
BiochemistryCompulsory Course1110

Class Information
Academic Year2017 – 2018
Class PeriodWinter
Faculty Instructors
Instructors from Other Categories
Weekly Hours3
Class ID
600107994
Course Type 2016-2020
  • Scientific Area
Course Type 2011-2015
Specific Foundation / Core
Mode of Delivery
  • Face to face
Digital Course Content
Language of Instruction
  • Greek (Instruction, Examination)
Prerequisites
Required Courses
  • ΜΧ031 Nucleic acids structure and function
  • ΜΧ032 Structure and function of proteins
  • ΜΧ033 Laboratory techniques in Biochemistry
Learning Outcomes
Upon successful completion of this course, students will come into contact with the research thinking and gain comfort in handling the tools needed to conduct biochemical research
General Competences
  • Apply knowledge in practice
  • Retrieve, analyse and synthesise data and information, with the use of necessary technologies
  • Make decisions
  • Work in teams
Course Content (Syllabus)
Techniques in Biochemistry laboratory Cultures of microorganisms and cells with/without plasmids and phages. DNA digestion with restriction endonucleases. Recombination in plasmid. Bacterial transformation with a plasmid. Ultra centrifugation for molecules separation. Amino acids analysis and determination of a peptide sequence. Electrophoresis of protein. Electro-trasfer of protein onto membranes. Immunodetection. Immunoprecipitation of proteins with antibodies and Protein A sepharose. Chromatographic technics. Determination of enzymatic activities with radioisotopes.
Keywords
Techniques, Biochemistry, Molecular Biology
Educational Material Types
  • Multimedia
  • Interactive excersises
Use of Information and Communication Technologies
Use of ICT
  • Use of ICT in Laboratory Teaching
Course Organization
ActivitiesWorkloadECTSIndividualTeamworkErasmus
Lectures1204.8
Laboratory Work1807.2
Total30012
Student Assessment
Student Assessment methods
  • Labortatory Assignment (Formative, Summative)
Bibliography
Additional bibliography for study
Molecular cloning laboratory manual. Third Edition. Sambrook and Russell
Last Update
15-10-2015