Learning Outcomes
Students is expected to have a well understanding of Mitosis and Meiosis and their importance in genetic analysis and evolution of the genomes of the cultivated species. To deeply understand the importance of chromosomal mutations and their use in genetic analysis. To obtain a good knowledge of the genetic variability that exists in alien related species and the methods applied in order to use this variability in breeding of the cultivated species.
Course Content (Syllabus)
Genetics & Evolution of cultivated plants.
Plant Genome & Mode of Reproduction.
Function of autosomes and of sex-chromosomes.
Cytogenetic structure and identification of chromosomes.
Handling and staining of chromosomes. Microscopy and Flow cytometry.
Mitotic cycle (S, G1, G2, M, C-value) and biological role.
Movement of chromosomes during mitosis and meiosis. Meiosis & gametogenesis.
Mutations and variation in chromosome structure. The role of deletions, duplications, inversions and translocations
on the normal function and evolution of organisms.
Polyploids, genome analysis and evolution.
Haploids in plant breeding. Aneuploids and genetic analysis. Gene location on chromosomes and chromosome arms. Linkage and chromosome mapping. Qualitative & Quantitative traits. Analysis of QTL's.
Interspecific and intraspecific hybrids.
Alien gene transfer through chromosome manipulation.
Parthenogenesis & Cytogenetic basis of apomixis.
Molecular cytogenetics (FISH, GISH, In SITU Hybridization).
Keywords
Mitosis, meiosis, chromosomal mutations, polyploidy, aneuploidy, genetic analysis, gene mapping, interpecific hybrids