Bioanalysis and -OMICS
| Title | Βιοανάλυση και τεχνολογίες -omics / Bioanalysis and -OMICS |
| Code | ΒΤ3 |
| Faculty | Engineering |
| School | Chemical Engineering |
| Cycle / Level | 2nd / Postgraduate |
| Teaching Period | Winter/Spring |
| Coordinator | Cristina Virgiliou |
| Common | No |
| Status | Active |
| Course ID | 600023202 |
Programme of Study: MSc Chemical and Biochemical Engineering: Health & Food
Registered students: 0
| Orientation | Attendance Type | Semester | Year | ECTS |
|---|---|---|---|---|
| KORMOS | Compulsory Course | 3 | 2 | 7.5 |
| Academic Year | 2025 – 2026 |
| Class Period | Winter |
| Faculty Instructors | |
| Class ID | 600245486
|
Course Type 2021
Specific Foundation
Mode of Delivery
- Face to face
Digital Course Content
- e-Study Guide https://qa.auth.gr/en/class/1/600245486
- eLearning (Moodle):
Language of Instruction
- Greek (Instruction, Examination)
- English (Instruction, Examination)
General Competences
- Apply knowledge in practice
- Make decisions
- Work in teams
Course Content (Syllabus)
- Introduction to Bioanalysis.
- Holistic analysis. omics techniques.
- Data processing and statistical analysis.
- Description of biological matrices.
- Sample preparation techniques.
- Metal-OMICS and applications.
- Modern analytical techniques in Bioanalysis and applications.
- Validation of analytical/bioanalytical methods.
1st Laboratory Exercise. Demonstration of a metabolomic analysis workflow using the LC-MS technique.
2nd Laboratory Exercise. Analysis of antibiotics in blood samples collected at different time points by LC-MS (available in the laboratory).
3rd Laboratory Exercise. Sample preparation using different matrices such as blood, urine, DBS and DUS for LC-UV and GC-MS analysis.
4th Laboratory Exercise. Quantitation of Copper in urine samples via FAAS.
5th Laboratory Exercise. Drug detection in biological samples by LC-qTOF and GC-MS/MS.
Educational Material Types
- Notes
- Slide presentations
- Interactive excersises
- Book
Use of Information and Communication Technologies
Use of ICT
- Use of ICT in Course Teaching
- Use of ICT in Laboratory Teaching
- Use of ICT in Communication with Students
Course Organization
| Activities | Workload | ECTS | Individual | Teamwork | Erasmus |
|---|---|---|---|---|---|
| Lectures | 24 | 0.8 | ✓ | ✓ | |
| Laboratory Work | 15 | 0.5 | ✓ | ✓ | |
| Reading Assigment | 183 | 6.1 | |||
| Exams | 3 | 0.1 | |||
| Total | 225 | 7.5 |
Student Assessment
Student Assessment methods
- Written Exam with Multiple Choice Questions (Summative)
- Written Exam with Short Answer Questions (Summative)
- Written Exam with Extended Answer Questions (Summative)
- Written Assignment (Summative)
- Performance / Staging (Summative)
Bibliography
Course Bibliography (Eudoxus)
Βιοαναλυτική Χημεία (2015), Γ. Θεοδωρίδης, Σ. Γηρούση, Γ. Ζαχαριάδης, Α. Ζώτου, Β. Σαμανίδου, Ελληνικά Ακαδημαϊκά Ηλεκτρονικά Συγγράμματα και Βοηθήματα, www.kallipos.gr .
Additional bibliography for study
1. Bioanalytical Chemistry, Mikkelsen and Corton, 2nd edition, Wiley, 2016.
2. FDA Bioanalytical Method Validation Guidelines: https://www.fda.gov/regulatory-information/search-fda-guidance-documents/bioanalyticalmethod-validation-guidance-industry.
3. Handbook of LC-MS Bioanalysis: Best Practices, Experimental Protocols, and Regulations, Wenkui Li PhD, Jie Zhang PhD, Francis L.S. Tse PhD.
4. Bioanalysis of Pharmaceuticals: Sample Preparation, Separation Techniques, and Mass Spectrometry, Steen Honoré Hansen, Stig Pedersen-Bjergaard.
5. The Handbook of Metabonomics and Metabolomics (2007), John C. Lindon, Jeremy K. Nicholson and Elaine Holmes.
6. Best Practice in Biological Sample Collection, Processing, and Storage for LC-MS in Bioanalysis of Drugs, Maria Pawula, Glen Hawthorne, Graeme T. Smith, Howard M. Hill.
7. Best Practices in LC-MS Method Development and Validation for Dried Blood Spots, Chapter 30, Jie Zhang, Tapan K. Majumdar, Jimmy Flarakos, Francis L.S. Tse.
8. Quantitative determination of endogenous compounds in biological samples using chromatographic techniques, Nico C.van de Merbel.
9. Glucose biosensors: an overview of use in clinical practice, Eun-Hyung Yoo, Soo-Youn Lee.
10. Metallomics: the concept and methodology, Sandra Mounicou, Joanna Szpunara and Ryszard Lobinski.
Last Update
20-02-2024
