Molecular Technology
| Title | Μοριακή Τεχνολογία / Molecular Technology |
| Code | ΙΑΒ03 |
| Faculty | Health Sciences |
| School | Medicine |
| Cycle / Level | 2nd / Postgraduate |
| Teaching Period | Spring |
| Coordinator | Georgios Tzimagiorgis |
| Common | No |
| Status | Active |
| Course ID | 600019987 |
Programme of Study: DtPMS Iatrikī Akriveías - Metafrastikī Éreuna kai THerapeutikī (2025-méchri sīmera)
Registered students: 0
| Orientation | Attendance Type | Semester | Year | ECTS |
|---|---|---|---|---|
| KORMOS | Elective Courses | 2 | 1 | 5 |
Programme of Study: PPS Precision Medicine-Translational Research and Therapeutics
Registered students: 4
| Orientation | Attendance Type | Semester | Year | ECTS |
|---|---|---|---|---|
| Core | Elective Courses | 2 | 1 | 6 |
| Academic Year | 2024 – 2025 |
| Class Period | Spring |
| Faculty Instructors |
|
| Instructors from Other Categories |
|
| Weekly Hours | 3 |
| Class ID | 600261851
|
Course Type 2021
Specific Foundation
Mode of Delivery
- Face to face
- Distance learning
Digital Course Content
- e-Study Guide https://qa.auth.gr/en/class/1/600261851
- eLearning (Moodle): https://elearning.auth.gr/course/view.php?id=14825
Language of Instruction
- Greek (Instruction, Examination)
Learning Outcomes
Learning the fundamentals of molecular biology techniques involving DNA, RNA and protein analysis and the role they play in investigating the molecular basis of genetic and epigenetic conditions and diseases.
General Competences
- Apply knowledge in practice
- Retrieve, analyse and synthesise data and information, with the use of necessary technologies
- Adapt to new situations
- Make decisions
- Work autonomously
- Generate new research ideas
- Be critical and self-critical
- Advance free, creative and causative thinking
Course Content (Syllabus)
Isolation of nucleic acids (DNA, RNA)
Quantitative and qualitative characterization/determination
Nucleic acid electrophoresis techniques
(types of gel, dyes)
Genetically engineered enzymes (restriction enzymes, modifying enzymes)
Mutation Analysis Methods (Theory)
Isolation of DNA or RNA, (practical part)
RT, RT-qPCR, (practical part)
Digestion with restriction enzymes, (practical part)
Agarose gel electrophoresis (practical part)
Gene expression analysis methods
Northern blot, primer extension,
RNA protection assay,
RT-PCR, RT-qPCR
Protein isolation-Quantitative determination
Protein separation and analysis techniques
Protein isolation, Quantification of total protein concentration (Bradford/BCA assay)
Preparation of polyacrylamide gel in the presence of sodium dodecyl sulfate (Sodium Dodecyl S-Polyacrylamide Gel Electrophoresis, 1D-SDS-PAGE)
Immunoblotting – Western Blot (Day 1) (practical part)
-Separation of proteins by gel electrophoresis in denaturing conditions (SDS-PAGE)
-General gel staining (Blue-silver staining)
-Liquid transfer of proteins from SDS-PAGE gel to PVDF membrane
-Blocking of non-specific membrane sites by incubation in milk or BSA solution
-Incubation of PVF with primary antibody (o/n)
Immunoblotting – Western Blot (Day 2) (practical part)
-Preparation of PVDF for incubation with secondary antibody
-Incubation with chemiluminescence reagent
- Appearance of antibody
-Problems during appearance and handling
-Process PVDF for incubation with subsequent primary antibody
(practical part)
Educational Material Types
- Notes
- Slide presentations
- Video lectures
- Interactive excersises
Use of Information and Communication Technologies
Use of ICT
- Use of ICT in Course Teaching
- Use of ICT in Communication with Students
- Use of ICT in Student Assessment
Course Organization
| Activities | Workload | ECTS | Individual | Teamwork | Erasmus |
|---|---|---|---|---|---|
| Lectures | 48 | ✓ | |||
| Laboratory Work | 90 | ✓ | ✓ | ||
| Written assigments | 8 | ✓ | |||
| Exams | 4 | ✓ | |||
| Total | 150 |
Student Assessment
Description
Postgraduate students are assessed with:
a) their oral participation in questions related to the understanding of topics presented in the lectures of the course (10%)
b) their effectiveness in carrying out the experiments/exercises during the practical part of the course (10%)
c) written exams involving searching for molecular biology information, solving laboratory exercises and answering questions (80%)
Student Assessment methods
- Written Exam with Multiple Choice Questions (Formative, Summative)
- Written Exam with Short Answer Questions (Formative, Summative)
- Written Exam with Problem Solving (Formative, Summative)
- Labortatory Assignment (Formative, Summative)
Last Update
01-03-2024
