NEXT GENERATION SEQUENCING
| Title | ΑΛΛΗΛΟΥΧΙΣΗ DNA ΝΕΑΣ ΓΕΝΙΑΣ / NEXT GENERATION SEQUENCING |
| Code | APBC.01.03 |
| Faculty | Sciences |
| School | Biology |
| Cycle / Level | 2nd / Postgraduate, 3rd / Doctorate |
| Teaching Period | Winter/Spring |
| Coordinator | Alexandros Triantafyllidis |
| Common | No |
| Status | Active |
| Course ID | 600022122 |
Programme of Study: DIPMS EFARMOSMENĪ VIOPLĪROFORIKĪ 2022
Registered students: 30
| Orientation | Attendance Type | Semester | Year | ECTS |
|---|---|---|---|---|
| KORMOS | Compulsory Course | 1 | 1 | 6 |
| Academic Year | 2025 – 2026 |
| Class Period | Winter |
| Faculty Instructors | |
| Instructors from Other Categories | |
| Weekly Hours | 9 |
| Total Hours | 28 |
| Class ID | 600289741
|
Course Type 2021
Specific Foundation
Mode of Delivery
- Face to face
- Distance learning
Digital Course Content
- e-Study Guide https://qa.auth.gr/en/class/1/600289741
- eLearning (Moodle): https://elearning.auth.gr/course/view.php?id=17265
Language of Instruction
- English (Instruction, Examination)
Learning Outcomes
Upon successful completion of the course, students will:
1) understand and process DNA sequencing data from different next-generation DNA sequencing platforms
2) become familiar with the most common next-generation DNA sequencing protocols
3) be able to analyze transcriptome, ddRAD and barcoding expression data
4) be able to perform statistical analysis of molecular pathways and gene ontologies in gene lists
5) become familiar with the above analyses in R, python and UNIX/Linux environments.
General Competences
- Retrieve, analyse and synthesise data and information, with the use of necessary technologies
- Work in an interdisciplinary team
Course Content (Syllabus)
Course contents include: 1) introduction to the nature and processing of DNA sequencing data (e.g. (a) the nature and processing of DNA sequencing data (e.g., filtering) from various next-generation DNA sequencing platforms; 2) theory and practical exercises on the most popular next-generation DNA sequencing protocols such as (a) hybrid sequencing of small and medium size whole genomes, (b) transcriptome synthesis, quantification, and expression analysis, (c) ddRAD, and (d) eDNA and barcoding, 3) gene characterization and statistical analysis of molecular pathways and gene ontologies in gene lists. A significant part of the practical exercises will be performed in R, python and UNIX/Linux environments.
Educational Material Types
- Slide presentations
- Video lectures
- Interactive excersises
Use of Information and Communication Technologies
Use of ICT
- Use of ICT in Course Teaching
- Use of ICT in Laboratory Teaching
- Use of ICT in Communication with Students
- Use of ICT in Student Assessment
Course Organization
| Activities | Workload | ECTS | Individual | Teamwork | Erasmus |
|---|---|---|---|---|---|
| Lectures | 90 | 3.6 | |||
| Field trips and participation in conferences / seminars / activities | 6 | 0.2 | |||
| Written assigments | 16 | 0.6 | |||
| Exams | 38 | 1.5 | |||
| Total | 150 | 6 |
Student Assessment
Student Assessment methods
- Written Exam with Multiple Choice Questions (Summative)
- Written Exam with Problem Solving (Summative)
- Report (Summative)
Last Update
28-01-2024
